Knowledgeable Volume 'High Resolution Chromatography: a Practical Approach' Provides the Necessary I

Jan 28, 07:24 AM

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules: oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

About the Author

Edited by Paul Millner, School of Biochemistry and Molecular Biology, University of Leeds

Content Outline:

A. Techniques and Equipment

1. Separation by charge, size, and hydrophobicity, Paul A. Millner

2. Practice and benefits of microcolumn HPLC, Frank J. Yang

3. Detection devices, Raymond P. W. Scott

4. Capillary electrophoresis of peptides and proteins, Bi-Huang Hu and Lenore M. Martin B. Selected affinity approaches

5. Lectins as affinity probes , Philip S. Sheldon

6. Nucleotide- and dye-ligand chromatography, Bo Mattiasson and Igor Yu. Galaev

7. Synthetic peptides as affinity ligands, G. J. Reynolds and Paul A. Millner

8. Drugs and inhibitors as affinity ligands , Nigel M. Hooper

9. Immunoaffinity chromatography, P. Cutler

10. Strategies and methods for purification of Canullnull-binding proteins, Sandra L. Fitzpatrick and David M. Waisman

11. Purification of DNA-binding proteins, H. Handa, Y. Yamaguchi, and T. Wada

For more information visit http://www.researchandmarkets.com/reports/c80952 Knowledgeable Volume 'High Resolution Chromatography: a Practical Approach' Provides the Necessary I
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